Boston College
Master of Science Candidate, Biology
Dr. Welkin Johnson
04/2015 - present

Developed and optimized an infectious system using an endogenous, or "fossilized," retroviral envelope found in the human and rhesus macaque genome in order to study the functional evolution of retroviruses.

Sanford Burnham Prebys Medical Discovery Institute
Research Assistant II
Dr. Sumit Chanda
07/2013 - 07/2014

Worked alongside scientists in identification of PQBP1 as a proximal sensor of HIV-1 DNA in the cGAS-dependent innate immune signaling. Also, worked in the HIV Immune Networks Team program in detecting early immune responses of HIV-1.

Genomics Institute of the Novartis Research Foundation
Scientific Associate I
Dr. Anthony Orth
07/2012 - 07/2013

Worked in collaboration with Albert Einstein University in creating knockout constructs  of M. tuberculosis. Also, doubled the production of DNA purifications for high-throughput.

Genomics Institute of the Novartis Research Foundation
Intern
Dr. Jonathan Deane
03/2011-09/2011

Maintained the cell lines and an orderly lab, conducted restriction enzyme cloning, and completed ELISAs.

 

Technical Experience

  • Cloning

    • PCR

    • Restriction Digests

    • Ligations

    • Transformations

    • DNA/RNA extraction

    • Electroporation

    • Gel electrophoresis

    • Gel extraction

    • Site-directed Mutagenesis

    • Reverse Transcription

    • cDNA Synthesis

  • Tissue Culture

    • Transfections

    • Infections

    • Virus Production

    • Viral Titers

    • Virus Purification

    • Cell line maintenance 

  • Analytic Techniques

    • Western Blot

    • ELISA

    • BCA

    • p24

    • qRT-PCR

    • Competition Assay

    • Plaque Assay

    • Phage display

  • Computation 

    • Lasergene Suite

    • Geneious

    • Database Integrated Genome Screening (DIGS)

    • SQL 

    • Microsoft Office Suite

    • Adobe Illustrator

  • Automation Experience

    • Hamilton Nimbus

    • Tecan Evo 

    • THEONYX

    • THEMATRIX

    • BenchCel

  • Optimization of experiments to maximize efficiency